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ISSN 2250 - 2033 (Print); e -ISSN: 2250 - 2041 (Online)


GTRP Journals


 International Journal of Applied Bioresearch

Volume  No. 38; 15,March, 2019; Current Issue


Volume No.38
Immunostimulant responses on Methanolic leaves extract on Syzygium caryophyllatum (L.) supplemented with basal diets fed on Vibrio parahaemolyticus infected Macrobrchium  idae (Heller, 1862)
Muthiah MARIDASS and Ganapathy RAJU
Department of Zoology, Pioneer Kumaraswamy College, (Affiliated to Manonmaniam Sundaranar University), Nagercoil,Tamil nadu-629003, India.
Corresponding Author Email maridassugcpdf@yahoo.co.in


The aim of the present study was growth performance and histopathological analysis of freshwater prawns of Macrobrchium idae (Heller, 1862) fed with supplemented diet on the different concentration of methanolic leaves extract of Syzygium caryophyllatum (L.) for 4 weeks. The experimental study groups were 0% (Control), 1,2,3 and 4% concentrations of methanolic leaves extract of S. caryophyllatum (L.) supplemented with control diet. The methanolic leaves extract of S. caryophyllatum supplemented with basal diet fed with normal and Vibrio infected M. idae for 28 days observed by growth parameters, histological analysis of hepatopancreas. The results were observed by excellent growth on weight gain (%), the survival rate (%) and FCR rate (%) of M. idea for 28 days. 4% of extract S. caryophyllatum (L.) leaves diet was observed by 100% of survival rate. Control fed with Vibrio parahaemolyticus infected prawn M. idae was observed 100 % of mortality within one week.  The control fed with M. idae was histologically observed by hepatopancreas is blind tubules, which is consisting of four type of cells. The E-cells at the summits of the tubules develop into R-cells (for resorption of nutrients), F-cells (for production of digestive enzymes) and B-cells (function unknown). Experimental diets fed with M. idae were observed by hepatopancreas was appeared to be healthy structure. The disease infected prawn M. idae fed with control diet was observed by unhealthy hepatopancreas conditions including slough hepatopancreas tubules cells, degeneration of tubules lumen, enlarged of the hepatopancreas nuclei cell and lack of B, F and R epithelial cells in the hepatopancreas tubules. The conclusion of the study shows that hepatopancreas conditions can be the indicator to determine the conditions of the prawn health status fed with all experimental diets. The conclusion of the present study may be acted as alkaloids, terpenoids, tannins, saponins, glycosides, flavonoids, phenolics, steroids and essential oils present in this plant. The plant extract of Syzygium caryophyllatum (L.) leaves was established in the potential use of additive for diets on freshwater prawn of M.idae.
Keywords: Freshwater, Macrobrchium idea (Heller, 1862), medicinal plants, bacteria, feeds, growth, pathology